SEROLOGICAL STUDIES ON THE FORMATION OF PROTEIN PARASPORAL INCLUSIONS IN BACILLUS THURINGIENSIS

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Serological Studies on the Formation of Protein Parasporal Inclusions in Bacillus Thuringiensis

A strain of Bacillus thuringiensis has been isolated, and methods have been developed for separation of the crystalline, parasporal inclusions in a pure form. Normal sporulation with concomitant crystal formation takes place when cells are incubated under suitable conditions in a nutrient free medium. Serological techniques have been used to study the origin and development of the crystals. Rab...

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Protein turnover and the formation of protein inclusions during sporulation of Bacillus thuringiensis.

During sporulation of Bacillus thuringiensiS, a spore and a protein parasporal inclusion are formed within each cell (Hannay & Fitz-James, 1955; Hannay, 1956). The parasporal inclusions are of a regular, dipyramidal form and are referred to as 'crystals'. Similar 'crystals' are formed by a number of related strains of Bacillus, all of which are pathogenic to insect larvae. The crystals are comp...

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Crystalline inclusions in Bacillus thuringiensis.

Crystalline inclusion bodies resembling those seen in Clostridium cochlearium were detected in cultures of Bacillus thuringiensis infected with bacteriophage.

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Characterization of the parasporal inclusion of Bacillus thuringiensis subsp. kyushuensis.

Electron microscopy of Bacillus thuringiensis subsp. kyushuensis revealed that the parasporal inclusions are composed of a homogeneous center surrounded by a thick, electron-dense coating. Antibodies directed against the 135- and 65-kilodalton B. thuringiensis subsp. israelensis peptides cross-reacted with the 70- and 26-kilodalton peptides, respectively, of B. thuringiensis subsp. kyushuensis.

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Poly-β-hydroxybutyrate Metabolism Is Unrelated to the Sporulation and Parasporal Crystal Protein Formation in Bacillus thuringiensis

Poly-3-hydroxybutyrate (PHB) is a natural polymer synthesized by many bacteria as a carbon-energy storage material. It was accumulated maximally prior to the spore formation but was degraded during the process of sporulation in Bacillus thuringiensis. Intriguingly, B. thuringiensis also accumulates large amounts of insecticidal crystal proteins (ICPs) during sporulation, which requires consider...

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ژورنال

عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology

سال: 1961

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.11.2.321